Der Pharmacia Lettre
Abstract
Author(s): Sandhya Desai, Bhaskar Gavitre, Mahesh Gite, Akbar Ahmad, Yogesh More,
Pooja Telang
The hepatoprotective effects of two polyherbal preparations viz., PHF-A and PHF-B containing
constituents with potential hepatoprotective activity were evaluated using Paracetamol-induced
hepatotoxicity. The treatment effect on lipid peroxidation (LPO) and levels of the associated
antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase
(GPX) were investigated in rats. In Paracetamol-induced hepatotoxicity liver damage was
induced in Wistar rats by administering Paracetamol (835 mg/kg i. p.) on 7th day. The standard
drug Silymarin (25 mg/kg), PHF-A (3 dose levels i. e. 200, 400 and 600 mg/kg) and PHF-B (3
dose levels i. e. 200, 400 and 600 mg/kg) were administered orally to the respective groups once
daily for 7 days. The levels of marker enzymes (SGOT, SGPT and ALP), and proteins [albumin
(Alb), globulin and total proteins (TP)] were assessed in serum. The effects of PHF-A on
antioxidant enzymes SOD, CAT, GPX and lipid peroxidation (LPO), were assayed in liver
homogenates to evaluate antioxidant activity. PHF-A (200 mg/kg), PHF-B (200 mg/kg),and
silymarin elicited a significant hepatoprotective activity by lowering the levels of serum marker
enzymes and lipid peroxidation and elevating the levels of SOD, CAT, GPX, Alb and TP in a
dose dependant manner. The present findings suggest that the hepatoprotective effect of PHF-A
and PHF-B in Paracetamol-induced damage might be due to inhibition of Cytochrome P-450
activity or prevention of inactivation of antioxidant enzymes.
