The research in the field of nanotechnology has grown rapidly over the past few years and has even ventured into the new field of therapeutic medicine. Of all kinds of nanoparticles, silver nanoparticles (AgNPs) seem to have attracted the most interests in terms of their potential application. In recent years, metal nanoparticles are explored in order to find a new approach to kill resistant pathogenic microorganisms. Silver nanoparticles (AgNPs) are one of the metal of choice for antibiotic resistance microbes. Synthesis of AgNPs through biological way is considered to be a green approach in the field of nanotechnology. In the present study, an eco-friendly process for the synthesis of nanomaterials using two fungal strains has been attempted. Two species of Aspergillus viz., A. ochraceus and A. sydowii were used for the biosynthesis of AgNPs. These AgNPs were characterized by UV–Vis spectroscopy, and Xray Diffraction (XRD). The nanoparticles exhibited maximum absorbance peak at 430 and 422nm in UV–Vis spectroscopy and the XRD analysis showed the diffraction peak of the values at 32, 38, 44, 46, and 64. In this study, the biosynthesis of AgNPs was carried out using two fungal species of Aspergillus isolated from coastal sand dune of Puducherry coastal area. A comparative study was done among these two species in order to analyze their nanoparticle characterization as well as the antibiotic efficacy against bacterial pathogens. The nanoparticle of both species showed antimicrobial activity towards certain gram positive and gram negative bacteria and also showed enhanced activity by combined with antibiotic Vancomycin.
