Silver nanoparticles are used to prevent and eradicate different bacterial disease are well known for long time. During recent scenario, bacteria mould themselves to resist varied antibiotics based on their peculiar characteristics and known as super bugs. In the present study, a supportive approach was carried out to synthesize silver nanoparticles (AgNPs) from two airborne allergenic fungi; Alternaria chlamydospora and Alternaria sp. The occurrence of yellowish brown color in the conical flask suggested the formation of AgNPs. The fungal culture filtrate with AgNo3 altered the solution into brownish color during the reaction. The silver nanoparticles were characterized by Uv-Vis spectrophotometry, Field emission scanning electron microscope (FESEM). Size of the nanoparticles measured between 20nm to 40nm by FESEM. The synthesized silver nanoparticles were subjected to their characterization by X-ray diffraction (XRD) technique to determine the metallic nature of nanoparticles. The pattern showed that silver nanoparticles have been formed resulting in the diffraction peaks in between 38 to 77 confirming the metallic nature of nanoparticles and peaks were specific for the silver nanoparticles. AgNPs synthesized from both Alternaria species showed good antimicrobial activity against the selected bacterial species, Bacillus cereus, Staphylococcus aureus, E. coli, Proteus vulgaris and Vibrio cholerae. The antibacterial efficacy of AgNPs combined with Oxacillin was found prominent in the study, the combined formulation of AgNPs of A. chlamydospora and Oxacillin was recorded with the best in comparison to the combined form between Oxacillin and AgNPs of Alternaria sp.
