A simple and accurate colorimetric method was developed for the estimation of paromomycin sulphate (PM) in bulk and pharmaceutical formulations. The method was based on generation of colored complex from reaction between PM and ascorbic acid having analytically useful maxima at 390 and 530 nm. The factors such as concentration of ascorbic acid and heating time affecting the color development were optimized and incorporated in the procedure. Regression analysis of Beer's plot showed good correlation (r=0.999) in a concentration range of 40-200μg/mL. The limits of detection and quanification at 390 and 530nm were 3.95μg/mL, 5.95μg/mL and 13.17μg/mL, 19.83μg/mL, respectively. The average recovery for the commercial preparation (Paromomycin Sulfate injections, 1g/ 2 mL) was 102.23±3.37%; n=3, which reflecte
