Cellulose as a degradable substance with hight abundant in nature constitutes moiety of gross weight product of green plant. The fungi are one of the major microorganisms to produce cellulase enzymes as β 1, 4 Glucanase, β-Glucosidase and Cellubiohydrolase. In this reaserch, the ability of CMC degradation of several soil fungi as Trichoderma, Aspergillus and Fusarium isolated from different region of Iran was investigated and characterization of cellulase from superior isolates. CMC (Carboxy Methyl Cellulose) is a basic liquid mineral medium as just carbon source were inoculated. Selected fungal inoculums in a randomised complete block design with three repetitions .Glucose and protein production assay were performed four days after inoculation and repeated each three days up to 30th day. The result of reaction of glucose with arsenat molibdat reagent was read with spectrophotometer at 525 nm and protein asay with Bradford metod was read with spectrophotometer at 595 nm. There were variations in measure glucose and protein, Glucose levels were increased to to 14th days and were decreased up to 25th days and were constant in the next days. Protein rate were increased to 9th day and were decresed to 25th day and it were fixed in next days. Our result showed that Trichoderma harzianum 15-6 isolate had highest and Fusarrium solani had least ability for CMC degradation and CMCase product in the experimental condition. The study clearly demonstrated that CMC is a good inducer for extracellular CMCase production by the fungus.
