In the present work, a comprehensive study was made on the analysis of chemical composition of essential oil and antibacterial activity from the leaves of Ajuga bracteosa. The essential oil was extracted by Cleavenger type apparatus and analyzed by GC-MS. The main components present in the essential oil of leaves are Limonene, α-humulene, β-Myrcene, Elemol, Camphene, β- Caryophellene, α-phellendrene.The antibacterial activity of the essential oil was evaluated. The essential oil was active against Staphylococcus . The plant extracts of Ajuga bracteosa was evaluated for antimicrobial activity. The methanol extract is acting against Staphylococcus aureas and acetone extract also active against E. coli The plant extract showed minimum inhibiting concentration against the bacterial strain e.g. E.coli, Bacillus, Pseudomonas and Staphylococos.
