This study was carried out to analyze the active constituents present in the flower of Spathodea campanulata (Bignoniaceae). Four compounds in ethanolic extract were identified by Gas Chromatography – Mass Spectrometry (GC-MS) analysis. Butane, 1, 1-diethoxy-3-methyl- (35.11 %) and n-Hexadecanoic acid (30.22%) were the major constituents of ethanolic extract. This is the first report of identification of active constituents from the flower of Spathodea campanulata by GC-MS.