Benzene extract was separated by column chromatography using silica gel as adsorbent. The column was eluted by different solvents in their increasing order of polarity. Compounds were isolated subsequently purified by repeated reverse phase, silica gel column chromatography and thin layer chromatography. Benzene fraction of Psidium guajava (leaves) yields three compounds in pure form i.e. 17-methoy-β-sitosterol (1), Cholest-5-ene-3, 25-diol (2), 1-hydroxy- 3acetoxy-7-methoxy-2’methyl-5’carboxy-(3-carboxy but-2-enyl)-furano (3’, 4’5, 6) - xanthone (3).Their structure were determined by spectroscopic and chemical methods.
