The hexane extract was separated by column chromatography using silica gel as adsorbent. The column was eluted by solvents in their increasing order of polarity. Compounds were isolated and purified by repeated silica gel, reverse phase silica gel, column chromatography and thin layer chromatography. Hexane fraction of Piper betle(leaf stalk) yields four aliphatic compounds in pure form i.e. Pentadecyl 6-hydroxytridecanoate(1), Pentatriacontanol, (2), Methyl hexacos-7-enoate (3) and 6, 9-heptacosa diene (4). Their structures were determined by spectroscopic and chemical methods.
