Bioassay guided isolation and characterization of hexane extract of folklore medicinal plants of India, yielded three aromatic active compounds. These are: thymol (1) from the seeds of Trachyspermum ammi, β-asarone (2) from the rhizomes of Acorus calamus and eugenol (3) from the flower buds of Syzygium aromaticum. Structures of the compound were established based on its physical and spectral data (UV, IR, 1H & 13C NMR and Mass) and cocomparison with an authentic samples. All compounds have been subjected for in-vivo hair growth studies and showed good to moderate activity when compared with control compound, minoxidil.
