Chemical investigation of the dichloromethane extract of Gracilaria tenuistipitata var. liui Zhang and Xia has led to the isolation of monogalactosyl diacylglycerol (1), β-sitosterol (2), linoleic acid (3), and 2-hydroxyethyl benzoate (4). The structures of 1-4 were identified by NMR spectroscopy.