A. stipitatum (Synonym: A. hirtifolium) or Persian shallot from Amaryllidaceae family with indigenous name of ‘‘Mooseer’’ is one of the important edible Alliums in Iran. It is native and endemic from northwestern to southern of Iran and grows as a wild plant in the Zagross Mountains at high elevations of different provinces with the climate of very cold to moderate cold. In this study, essential oil constituents of A. stipitatum were investigated through gas chromatography/mass spectrometry (GC-MS) technique. After obtaining the chromatograms and mass spectra, the essential compounds of the essential oil were determined. As shown 1-Butene,1-(methylthio)-(Z) (18.21%), Methyl methylthiomethyl disulfide (8.41%), Dimethyl tetrasulfide (6.47%), Piperitenone oxide (4.55%) were the most abundant components and comprised 37.64% of the essential oil (Figure 1). While 2,5-Diethylthiophene (0.07%) and n-Nonanal (0.06%) were detected in lower amounts. The presence of compounds showed mono-sulfur (22.42%), disulfide (1.81%), tri-sulfur (13.57%) and tetra-sulfur compounds (6.47%). The results indicated that the highest amount of sulfur compounds is related to mono-sulfur compounds.