Species belonging to the genus Serjania are found mainly in regions of tropical climate. Studies described in the literature report the presence of alkaloids, saponins, flavonoids, coumarins, diterpenes and catechins. However, few studies describing the chemical composition for species of this genus are found in the literature. Herein we report the isolation of the flavonoids quecetin 3-O-β-D-glycoside and rutin as well as the saponin Pusatilla D.