The hemp ( cannabis sativa ) seed oil is extracted from seed. It was examined for physical and chemical properties. .It was analysed by Percentage ash, Refractive index, Specific gravity, Acid value, Iodine value, Saponification number, Unsaponifible mater content and Peroxide number. Hemp seed oil contains fatty acids that maintain healthy blood vessels , nerves and tissues. The high performance liquid chromatography ( HPLC ) is a suitable analytical method for seed oil of hemp. It was detected by HPLC chromatogram showing retention time.
