Two hundred and four fish species belonging to 34 families were collected from natural water bodies of downstream Subansiri river drainage. Cyprinidae found to be the dominant family with 72 species. Bagridae, Sisoridae, Erethistidae, Nemacheilidae, Cobitidae, Channidae, Schilbeidae, Osphronemidae and Siluridae were the other species rich families containing five to sixteen fish species. The collection contained 4, 5 and 22 fish species belonging to Endangered, Vulnerable and Near Threatened categories respectively. Further, 16 Data Deficient fishes were also collected during the study. Accumulation of such a large number of fish species from a single river is rare in entire Indian subcontinent and elsewhere. The checklist of fishes presented herein will serve as a database for future management of fishes in Northeastern region of India.