European Journal of Zoological Research
Abstract
Author(s): Sasan Hamzei,Milad Anvarian,Maryam Pouryusef Miandoab, Ghasem Yekani Tachinabad,
Hamed Moomivand and Ali Zali-Moghadam
Lactobacilli bacteria are gram positive non-spore forming, catalase-negative and filamentous bacteria that mainly
ferment different carbohydrates into lactate and acetate. Fifty perch (perca fluviatilis) hunted from Aras Dam, Iran,
randomly. The samples were transported to the Microbiology Laboratory, under sterile conditions in the vicinity of
ice and after dissection according to sterile method 1 gram of stool was sampled from the anterior part of intestine
and cultured in MRS agar-plates. After phonotypical and biochemical identity of bacteria, for distinction of
lactobacillus species molecular characterization, 16S rDNA gene was amplified and PCR products were sequenced.
Results of sequencing about 3 isolated bacteria indicated that this species has been Lactobacillus plantarum and the
founding�¢����s corresponding to produce results from biochemical tests and determine cutting pattern for this species.