The global demand for gelatin has been increasing over the years, because it has broad applications in the food, pharmaceutical, photographic, cosmetic and packaging industries. In the food industry, gelatin is used as an ingredient to enhance the elasticity, consistency and stability of food products. It is also used in some medical and biotechnological applications. So, the present study aims to extract fish gelatin from the fish processing waste, i. e skin of Black spotted Croaker (Protonibea diacanthus). The qualitative and quantitative parameter includes proximate analysis (viz. moisture, fat, protein, ash and pH), viscosity, color, Hydroxyproline content and electrophoresis studies were done on the extracted fish gelatin from skin of Black spotted Croaker. It was found that, the extracted fish gelatin is well suited to exploit gelatin, which can be used in the food industry. Ghol fish skin has been recognized as a good source of high quality collagen that can be employed to manufacture functional food, medicine and cosmetic products.