This research evaluated effect of different plant culture media on callus induction on Pluchea lanceolata. P. lanceolata is an important medicinally plant species that is rich in secondary metabolites like quercetin, β-sitosterol, triterpenol, etc. Four different types of culture media were employed to determine callus responses viz. MS1 (Murashige and Skoog medium), MS2 (Woody Plant medium), MS3 (Wood & Braun medium) and MS4 (White’s medium). Each medium was supplemented with 6-Benzyladanine (BA; 2 mg L-1) and α-Naphthalene acetic acid (NAA; 2 mg L-1) and tested for their influences. There are differences in callus initiation and morphology among the media. MS1 medium showed better responses in terms of fresh weight, dry weight, water content and chlorophyll content as compared to other medium used.
