A curcumin method has been used to measure the Boron concentration in water. Forty samples has been taken from Euphrates and Tigris rivers in Thiqar Governorate (southern of Iraq).The Boron levels for Euphrates river ranging between 1.729 mg/l and 0.476 mg/l with average value 0.794 mg/l , while for Tigris river ranging from 0.410 mg/l to 0.171 mg/l with average value 0.244 mg/l. The results shows that Boron concentration in Euphrates river is larger than that’s of Tigris river in general. Al Nasriya(2) shows the maximum Boron concentration with 1.729 mg/l. All samples of water were collected during Jun 2014 .