Synthesis of silver nanoparticles is achieved through methods such as physical, chemical and biological. Biological method of nanoparticles synthesis is economical and eco-friendly. The plant extract of DaturaalbaNees. incubated with silver nitrate showed gradual change in colour from colourless to reddish brown with increasing intensity. UVVisible spectrophotometry revealed that peak was obtained at 444 nm. X-Ray Diffraction analysis has shown that silver nanoparticles were crystalline and domain particle size was 28.42 nm. Scanning Electron Microscopy analysis showed aggregates silver nanoparticles and these were spherical in shape. These phytosynthesized nanoparticles were tested for their antibacterial activity. Antibacterial activity of silver nanoparticles was analyzed by measuring the inhibitory zone. It was observed against Chlostridiumdiptheriae.
