Present paper describes a rapid and eco friendly, non toxic and cost effective approach for green synthesis of Copper nanoparticle (CuNPs) using Vitis vinifera (Black and Green Grapes) leaf extract. The synthesized Copper nanoparticles were characterized by UV Visible Absorption Spectrophotometer, X-Ray Diffraction (XRD) and Fourier Transform Infrared (FTIR). The X-Ray diffraction analysis showed that the Copper nanoparticles are crystalline in nature with average 3 – 6 nm in size. The synthesized Copper nanoparticles showed effective activity against Escherichia coli and Bacillus subtilis.
