The biological synthesis of nanoparticles materializes as an eco-friendly and stimulating approach in the field of nanotechnology. In the present investigation, we report the extracellular biosynthesis of silver nanoparticles (AgNP`s) using entire plant extracts (Mollugo nudicaulis) for the reduction of aqueous Ag+ ions and its increased antimicrobial activity. Stable silver nanoparticles were formed by treating aqueous solution of AgNO3 with the plant extract as reducing agent for reduction of Ag+ ions. The quantitative formation of synthesized nanoparticles examined by Ultraviolet-Visible (UV-Vis) spectroscopy, Fourier Transform Infrared spectroscopy (FTIR) and X-ray diffraction (XRD). After the characterization, these nanoparticles are monitored for the antimicrobial activities of antibiotics (Tetracycline), silver nanoparticles and the combined effect of both antibiotics and nanoparticles against clinically isolated organism. The antibacterial activities of Tetracycline increased in the presence of Ag- NPs against Vibrio cholerae.
