Bacillus cereus is a highly underestimated endospore forming food borne bacterial pathogen that is ubiquitous in nature. Though reported from milk and various milk products marketed in India, no study was there to characterize isolates from fresh raw chhana (coagulated milk separated from whey) which is used to produce various sweets. Its presence in milk and dairy products is a matter of concern in food industry because of its spoilage and pathogenic potential. Two different types of toxins are produced by B. cereus causing diarrhoeal and emetic syndrome. In this study B. cereus was isolated from fresh raw chhana from sweet meat shops in and around Kolkata. Seven of the isolates were studied for their extracellular enzyme production, antibiotic resistance pattern and biofilm forming potentiality. All the isolates produced amylase and caseinase but none produced lipase. All the isolates were multiple-drug-resistant. Three of the isolates showed positive result for biofilm production by three different methods. Presence of multiple-drug-resistant bacteria with biofilm forming potentiality poses threat to the public health
