Andropagon gayanus (Gamba grass) was assessed for Bio-ethanol production potentials. The grass was hydrolyzed using 5% and 10% sulphuric acid and fermentated using cultured bacteria (Zymomonas mobilis) and cultured yeast (Saccharomyces cerevisiae). It was found that the percentage yield of the ethanol in the distillate obtained from the process involving cultured bacteria (Zymomones mobilis) was 26.50% from 5% H2SO4 and 44.90 % from 10% H2SO4. For the process involving cultured yeast (Saccharomyces cerevisiae) the values were 35.80% from 5% H2SO4 and 35.30 from 10% H2SO4.