Considered to be the cleanest liquid fuel, bio-ethanol can be a reliable alternative to fossil fuels. It is produced by fermentation of sugar components of plant materials. The Ajowan plant is considered to be a favorable source of fermentation products as they have sugar contents as well as contain various nutrients. This study focused on the effective production of ethanol from Ajowan plant by the yeast “Saccharomyces cerevisiae” for the first time. The results showed that the total sugar concentration of Ajowan plant was 68.4 g/l. The maximum rate of productivity, ethanol yield and final bioethanol percentage was 8 g/l/h (g ethanol per liter of Ajowan plant per hour), 35 g/l (g ethanol per liter of Ajowan plant) and 76%, respectively.
