An attempt has been made to assemble the biodiversity of spider fauna near Narmada River at Rajghat (Barwani), M.P. There are 159 spiders was collected from the Rajghat (Barwani) near the Narmada basin, out of which 50 species were identified belonging to 12 family namely; Araneidae (17), Salticidae (11), Oxyopidae (3), Erasidae (4), Therididae (3), Thomosidae (2), Lyosidae (3), Pholcidae (2), Clubiondiae (1), Philodromidae (1), Tetragnathidae (2) and Uloboridae (1).The most abundant species were represented by the families, Araneidae and Salticidae. Its identification was done on the basis of morphometric charaters of various body parts.