The diversity of actinomycetes in the Manakkudi mangrove ecosystem were analysed by the present study reveals that, the diversity of actinomycetes are found maximum in the rhizosphere soil than the non-rhizosphere soil that too mangrove associate of Achrostichum aureum harbours maximum counts than true mangrove plants. The diversity of actinomycetes is found maximum between the soil depth of 10-20cm are not correlated with the maximum level of nutrients between the soil depth of 0-10cm. The presence of actinomycetes in the Manakkudi mangrove ecosystem could pave the way for the establishment of disease free mangrove seedlings in the nursery and in the field. Efforts have been undertaken to find out the bioactive potential of mangrove sediment actinomycetes for the treatment dreadful human diseases.