Rosaindica and Psidiumguajava methanol extracts were tested for their effect on urease and collagenase enzyme by using phenol hypochlorite method and gelatin digestion assay, respectively. Among the two plant extracts evaluated, the highest inhibitory activity against Jack bean urease was exerted by R. indica(47.11%), while for collagenase P. guajava showed complete inhibition of enzyme at concentration of 10 mg/ml. Both the R. indica and P. guajava extracts showed potent urease (IC50 value: 1.42 and 2.08 mg/ml) and collagenase (IC50 value: 7.29 and 4.10 mg/ml) inhibition activity. The GC-MS analysis provided different peaks of twelve compounds ofR. indica and twenty three of P.guajava. Quinic acid (43.12%),Pyrogallol (21.92%), and 5-hydroxymethylfurfural (11.52%) were the major compounds in R. Indica while, Pyrogallol (27.64%), Isogeraniol (15.41%), and 1-Butanol, 3-methyl (10.92%) were found in P. guajava. R. indica and P. guajava extracts exhibited potent inhibitory activity against urease and collagenase enzyme, which could be attributed to the presence of various bioactive constituents identified by GCMS. The findings of our study endorse the use of these plants for further studies to determine their potential in management of pathologies caused by tested enzymes.
