Asymptomatic Urinary tract infection (UTI) is the presence of significant number of bacteria in a clean catch mid stream urine of an individual without symptom. 600 school children comprising of 350 females and 250 males aged 4-12 form three primary schools in Onicha Local Government area of Ebonyi state were randomly selected to determine the prevalence of urinary tract infection using > 104 colony forming unit per mill liter of urine as significant level of bacteria the prevalence was for a to be 48% (286). There was no significant difference between age and rate of infection (P > 0.05), Staphylococcus aureus was most frequently isolated (43.6%) followed by E. coli (16.0%) Klebsiella pneumoniae (11.6%). Enterococcus faecalis (9.4%), coagulase negative Staphylococcus (8.8%) Pseudomonas aeruginosa (6.7) Streptococcus pyogenes (1.9%) and Proteus mirabilis (9.0%). This finding underscores the need for screening of children regularly to prevent cases of asymptomatic UTI from becoming symptomatic with consequential harm.