Total 20 and 15 fungal species were isolated from fresh and market root samples of drug Aegle marmelos Corr. In the fresh samples, F. solani with 22.25% and in market samples A. niger with 26.97% recorded in high percentage incidence. Root samples were stored under different 30, 50, 75, 96 and 100 % relative humidity and different incubation days 15, 30, 45 and 60 days. Maximum growth and occurrence incidence of fungi were observed under high relative humidities 75, 96 and 100% RH and long storage periods 45 and 60 days. Quantitative estimation of alkaloids and glycosides in association with isolated fungi was done. Rate of deterioration of chemical constituents in the samples were noted at above 75% RH. Analysis of variance also showed that the effect of relative humidity and incubation days on biodeterioration of these chemical constituents were significant at 5% level of significance.