We evaluated the antimicrobial activity of crude extracts of alkaloids in seeds and roots of the plant Peganum harmala L. against some gram positive bacterial strains such as Staphylococcus aureus and Staphylococcus saprophyticus and gram negative such as Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis and Serratia spp, using the diffusion method in agar (antibiogram). Crude extracts of roots and seeds alkaloids have shown considerable activity against all organisms tested, seeds alkaloid showed a high activity at a concentration of 100mg/ml compared to roots alkaloidal at the same concentration, especially against Gram+ strains. The diameters of inhibition zones ranged from 11 to 22 mm for all treatments. The values of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. It was observed that the MIC and MBC of crude alkaloid extracted from the seeds Peganum harmala reached 0.78mg/ml, with the bacterial strain S.aureus (ATCC25923).
