The aim of this study was to assessed the alteration in biochemical parameters in Theileriosis infected cattle’s and buffaloes in Ahilyamata Gaushala (Kesharbag) of Indore District (M.P.). This study has been carried out during rainy season. It has been noticed that blood glucose content was significantly decrease (P<.01) in both T. annulata infected cattle and buffaloes as compared to normal. Whereas, AST/ SGOT, ALT/ SGPT and serum creatinine was found to be markedly increase (P<.01) in both T. annulata infected cattle and buffaloes compared to normal in Ahilyamata Gaushala (Kesharbag) of Indore. Results indicates that this alteration in biochemical parameters may creates various kinds of dysfunction in studied animals which causes huge economic loss tolerated by farmer society in studied region of Indore District.