Der Pharmacia Lettre
Abstract
Author(s): Nishitkumar S. Patel, Vrijeshkumar P. Gandhi, Rajendra S. Mehta,
Kashyap K. Bhatt
Simple and accurate liquid chromatographic (HPLC) and thin layer chromatographic (HPTLCdensitometry)
methods for simultaneous determination of camylofin dihydrochloride (CAM) and
diclofenac potassium (DIC) in tablets were elaborated. The first method was based on isocratic
reversed phase liquid chromatography by using RP ODS-C18 column with a mobile phase
consisting of acetonitrile:25mM potassium dihydrogen phosphate (80:20, v/v) containing 0.1%
v/v acetic acid adjusted to pH 7 with triethylamine at a flow rate of 1.5 mL/min and UV detection
at 215 nm. The calibration curve of camylofin and diclofenac was linear in the range of 50-300
�Ž�¼g/mL (r>0.999) and 5-30 �Ž�¼g/mL (r>0.999), respectively. The second thin layer
chromatographic method employed by using pre-coated silica gel G60Ã?¢Ã?â?¬Ã?â??F254 aluminum sheet
using mobile phase chloroform:ethyl acetate:methanol:ammonia (5:3:2:0.1,v/v) and quantitation
was achieved using spectrodensitometrically at 215 nm. The calibration curve of camylofin and
diclofenac was linear in the range of 1500-9000 ng/spot (r>0.999) and 150-900 ng/spot
(r>0.999), respectively. The validity of the methods was confirmed using the recovery studies,
precision and limit of detection. Both techniques were applied successfully for the analysis of
camylofin and diclofenac in tablets form. The results obtained from both procedures were
statistically compared using the Student�¢����s-t and F-variance ratio tests.
