Panchagavya is an incredible source for many medicinal substances. It has been reported for synergistic action but their scientific data are not available. Sixty mice were randomly divided into ten groups. The first, second, third, fourth , fifth , sixth, seventh, eighth, ninth & tenth groups received PG 1, PG1+ EEAB 10%, PG1+ EEAB 50%, PG1+ EEAB 75%, PG2, PG2+ EEAB 10%, PG2+ EEAB 50%, PG2+ EEAB 75%, Standard Alprazolam, Control Urine every day administered at the dose 4ml/kg body weight regularly at 9:00 am for 21 days & investigated the role of different composition of Panchagavya and its ethanolic extract of Aloe barbedansis Mill (EEAB) (Xanthorrhoeceae) for synergistic Anti-stress activity by using Forced Swimming Method in Swiss albino mice.On the 1st , 6th , 11th , 16th & 21th day after drug administration, effect of PG 1 , PG 2 , PG 1 + EEAB and PG 2 + EEAB were found to be significant at the level p<0.01 as compared to Standard and Control group. The synergistic activity of PG with EEAB might to be due to enhanced brain Gamma Amino Butyric Acid (GABA) level of mice and decreased brain dopamine levels & plasma corticosterone levels.
