The antioxidant and the antimicrobial activities of flavonoids extracted from Thymus ciliatus (Desf.) Benth., an endemic species in Algeria were investigated. The radical scavenging capacity using 2,2- diphenyl-1-picryhydrazyl (DPPH) and the reducing power test were determined to evaluate the antioxidant activity of the samples. The antimicrobial activity was tested with three bacterial strains and three fungi including yeast (Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 13311, Staphylococcus aureus ATCC25923, Aspergillus niger 2CA936, Aspergillus flavius NRRL3357 and Candida albicans ATCC1024). Our results showed a very strong antioxidant activity and an interesting antifungal power.