In this study, pomace olives coming from different mills (Press process, continuous process two-phases and threephases) were used for extraction of oils. The highest oil yield (12.92%) was obtained with pomace olives coming from press process. Total contents of phenolics (13.47 - 21.25 mg GAE/100 g oil) and flavonoids (5.90 - 12.52mg QE/100 g oil)were determined spectrophotometrically. The pomace olive oil “POO3” (Pomace olive coming from 3-phases system) presented the highest phenolic, flavonoid contents and showed the highest DPPH, ABTS scavenging, metal chelating activity. In vitro anticholinesterase activity, the olive pomace oils showed moderate inhibition against AChE and BChE which are the key enzymes taking place in pathogenesis of Alzheimer’s disease. These results showed that the tested oils can be considered as sources of natural antioxidant, as well as moderate anticholinesterase agents.
