The last decades are marked by the special interest in the development of medicinal plants at interest as a source of bioactive natural. As a result, many studies focus, increasingly, the therapeutic effects of natural antioxidants. In this context, we are interested in the study of antioxidant phenolics compounds, including flavonoids, capacity of a medicinal plant extracts (Ecballium elaterium L.). The plant has presented mean levels of polyphenols and flavonoids. Estimation of antioxidant activity of organic fractions was assessed by three methods: the reduction of iron, the DPPH free radical scavenging and hydrogen peroxide scavenging. The results showed that the polyphenols (including flavonoids) exhibit average antioxidant activity compared with reference antioxidants.