Tylophora. indica (Family: Asclepiadaceae) have long been used for the treatment of asthma, bronchitis, whooping cough, dysentery, rheumatic gouty pains and hydrophobia .The present study was conducted to evaluate the antioxidant activity of the aqueous and alcoholic leaf extract of an endangered medicinal plant T. indica. The antioxidant potential of the aqueous and alcoholic leaf extract of T. indica (native and micropropagated) was evaluated in vitro by employing the LPO, CAT and SOD assay. Results showed that aqueous extract is better antioxidant than alcoholic extract. The results of in vitro experiments revealed that the leaf extract of T. indica (AQTL) resulted in increase in the catalase and superoxide dismutase activity and decrease in LPO level, which were comparable to those of L-ascorbic acid. Hence, it is concluded that T. indica leaves has antioxidant activity and can be further employed for medicinal applications.
