Hymenocallis littoralis (Jacq.) Salisb. (Amaryllidaceae) commonly known as Beach spider lily, is an ornamental and medicinal plant, traditionally used for wound healing. It is used as an emetic and has shown anti-neoplastic, cytotoxic and anti-viral properties. The plant is folk remedy to treat freckles and blemishes. The aim of this research is to explore the anti-oxidant potential of this selected plant material. Two successive and two crude extract of its flowers were subjected for in-vitro anti-oxidant activity by savenging of hydroxyl radical in p-NDA method. The successive ethanol extract have shown potent anti-oxidant activity by p-NDA method with 71 ± 0.011, 79 ± 0.011, 56 ± 0.011, 54 ± 0.011 (mean ± S.E.M) for 10 μg/ml, 20 μg/ml, 40 μg/ml and 80 μg/ml, respectively. The crude ethanol has shown significant anti-oxidant activity by scavenging of hydroxyl radical in p-NDA method. All the concentrations prepared were paving dose dependent anti-oxidant activity.
