Urinary tract infection (UTI) is one of the most common microorganisms infections encountered by clinicians in developing countries. Therefore, the present research was carried out to determine the antimicrobial susceptibility pattern of microorganisms causing urinary tract infections. This cross sectional study was carried out between January 2013 and June, 2014 in Oyo State, Nigeria. Out of 840 urine samples collected using the mid-stream “clean catch” method. Antimicrobial susceptibility test was performed for the isolated pathogens using Kirby-Bauer disk diffusion method according to clinical and laboratory standards institute guidelines. Of these samples 584 (69.5%) were reported to be positive with gram negative, gram positive and yeast with the following 414 (70.9%), 7122 (20.8%) and 48 (8.2%) respectively. Uropathogens most frequently isolated were E.coli (29.6%), K.pneumoniae (17.5°/), P.mirabils (14.4%0, P.vulgaris (5.8%), P.aeruginosa (3.3%), Staphylococcus aureus (5.0°/), Enterococcus faecalis (9.4%), Coagulase-negative staphylococcus aureus (6.5%) while yeasts were (8.2%). E.coli and K.pneumoniae showed the highest percentage of resistance to ampicillin and Gentamycin (98%) however, S.aureus and coagulase negative staphylococcus were resistant to ampicillin (100%) while all the gram positive organisms were susceptible to vancomycin and Teicoplanin. As drug resistance among microorganisms pathogens is an evolving process, routine surveillance to provide physicians knowledge on the updated and most effective diagnosis treatment of UTIs.
