Euphorbia milii (Euphorbiaceae) is an ornamental plant plays a role in folk medicine. The Chinese use it as a cure for cancer, and some Brazilians believe that it can cure warts. The plant material of Euphorbia milii was collected from Kadiyapulanka, Rajahmundry, East Godavari District, A.P, India, in September 2014.The Antimicrobial activity studies of hexane, ethyl acetate, acetone, methanol and water extracts of flowers of Euphorbia milii (Euphorbia) were performed on gram positive organisms, Bacillus subtilis, Staphylococcus aureus, and gram negative organisms Escherichia coli and Proteus vulgaris by using cup plate method. The hexane, acetone and methanol extracts in the concentration of 5μg/ml, have shown considerable inhibition zone on Staphylococcus aureus& Bacillus subtilis as compared to other extracts. The hexane, acetone and methanol extracts in the concentration of 5μg/ml, have shown considerable inhibition zone on Escherichia coli & Proteus vulgaris as compared to ethyl acetate and water extracts which was compared with the inhibition zone produced by standard amikacin sulphate (1μg/ml.The estimation of phenolic compounds of hexane, ethyl acetate, acetone, methanol and water extracts of flowers of the plant Euphorbia milii (Euphorbia) were determined with the Folinciocalteu’s reagent (FCR) by UV-Visible Spectrophotometric method. The concentrations of the extracts were selected as 2μg/ml, 4μg/ml, 6μg/ml, 8μg/ml and 10μg/ml, after performing the assay the results indicating that hexane and acetone extracts contain more amounts of phenolic compounds than other extracts. These studies, indicates that the plant Euphorbia milii (Euphorbia) contains phenolic compounds which can act against selective gram positive and gram negative organisms.
