This study carried out to assay for the activity of the phytochemical properties as well as Gomphrena celosioides (GC) against Staphylococcus species a prominent bacterial agent associated with vulvovaginitis. A total of 100 samples of high vaginal (HVS) were collected from women of reproductive age 15-50 years for suspected vaginitis at Ahmadu Bello University, Health Care Center and Samaru Clinic, Zaria, Kaduna State, Nigeria and investigated for the presence of Staphylococcus species and the effect of GC extracts on the isolates. A total number of 74(74%) were found to be infected with Staphylococcal vaginal infection. The result of the ten randomly selected Staphylococcus spp using Microgen kit also showed that S. xylosus and S. aureus and Staphylococcus spp were present in 3 samples, 3(1%), 3(1%) respectively. S. warneri 1(0.3%). The methanolic and aqueous extracts of the plant contain secondary metabolites such as alkaloids, flavonoids, saponins with the absence of phenols, anthraquinones and steroids. All the clinical isolates of the organism were sensitivie to ciprofloxacin, amoxicillin and Erythromycin with the exception of isolate S. aureus (c) and Staph sp(c) which were resistant to amoxicillin. At a concentration of 1000mg/ml, the methanolic extract exhibited appreciable inhibitory activity on most of the isolates with a zone of 20mm and 19.33mm for S. aureus and Staph. sp respectively. The aqueous extract with the same concentration was less effective showing activity on S. xylosus (a) and (c) with zones of 10.00mm and 10.33mm respectively
