This work describes titanium dioxide (TiO2) nanoparticle coating on surface modified High density polyethylene films (HDPE) films and verifying the antimicrobial activity of the coated films against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Ultrasonication method was employed for coating the TiO2 nanoparticles on polymeric film and presence of TiO2 nanoparticles on the coated films was confirmed by X-ray diffraction (XRD), Scanning Electron Microscopy (SEM) And EDAX. Zone inhibition method was used to verify the antimicrobial activity of the coated films. This developed coating process is observed to render the HDPE film, a reasonable level of antimicrobial activity against the said microorganisms.