Bacopa monnieri L. (family Scrophulariaceae) is a traditional medicinal plant in India, known locally as ibrahmi. In the current study, the methanolic extract of the leaf callus of Bacopa monnieri at the concentrations o.25, o.o5, o.o3 mg/disc was investigated for its antimicrobial activity by modified Kirby-Bauer diffusion method using ciprofloxacin and griseofulvin as antibacterial and antifungal reference standard respectively. The finding of this study revealed that the extract of the leaf callus of Bacopa monnieri possessed a dose dependent antimicrobial activity against all the tested bacterial and fungal species indicated by the zones of inhibition of the microbial growth. The antimicrobial effect of the plant callus under investigation was comparable with those of the reference standards.
