Leaves of Sphaeranthus indicus were extracted with ethanol. The antimicrobial activities of Ethanolic extracts were evaluated by using in vitro disc conc. of 1.25, 2.5 and 5mg/disc. The minimum inhibitory concentration was calculated using broth tube dilution method. Significant antibacterial and antifungal action was observed in Ethanolic extract of leaves against B.subtilis, S.aureus and Candida respectively. The MIC of B.Subtilis and S.aureus were 0.5 and 2.5/ml and MIC of Candida and Aspergillus niger were 0.5 and 5mg/ml respectively
