In this study, we reported the antibacterial activity of silver nanoparticles. Here the nitrogen fixing bacterium Rhizobium species was isolated from rhizosphere soils of agricultural lands using spread plate technique. The pure isolates were inoculated in liquid broth and incubated in appropriate condition for the production of reducing agents. The extracellular components of those bacteria were separated from broth and used to reduce 1mM silver nitrate into silver nanoparticles. The reduction was initially confirmed by UV-Spectrophotometer size and agglomerations were determined by AFM analysis. The Antibiogram pattern of silver nanoparticles were expressed by disc diffusion method and it showed the silver nanoparticles exhibits increased antibacterial activity when combined with commercial antibiotics.
