The principal objective of the present research work was to determine the antibacterial potential of A. marmelos against four standard pathogenic bacterial strains. To evaluate antibacterial activity the agar-well diffusion assay was used. All the four extracts showed the highest and significant antibacterial activity against both Gram negative and Gram-positive bacteria. It is the strain Bacillus subtilis that is almost resistant to the four extracts of A. marmelos.