Plants have potent components of phytomedicine. Plant based natural constituents can be derived from any part of the plant like bark, leaves, fruits, flowers, roots, seeds etc., The therapeutic use of medicinal plant is becoming popular because of its inability to cause side effects and combat antibiotic resistant microorganisms. Medicinal plants such as Psidium guajava, Punica granatum, Prosopsi juliflora and Aegle marmelos are important sources of antibacterial, antifungal and antioxidant compounds. These plants contain secondary metabolites such as alkaloids, flavonoids, steroids, phenolics, terpenes, volatile oils etc., These are important for radical scavenging effects as well as their potential antibacterial, estrogenic and anti- cancer activities. The objective of this research is to determine the antibacterial, antifungal and phytochemical analyses of the four medicinal plants. Punica granatum was more effective followed by Prosopis juliflora, Psidium guajava and Aegle marmelos.