The chemical composition of the hydrodistilled essential oil of Satureja calamintha ssp. sylvatica, collected from Jijel (Eastern Algerian), was analyzed by GC and GC/MS. 23 components representing 94.45% of the essential oil were detected with isopulegone (66.5%) and pulegone (11.6%) as the major components. The antibacterial activity of the essential oil was tested against 10 gram-positive and gram-negative bacteria by the use of the disc diffusion method.