The antimicrobial activity of Ethanolic extract of the whole plant of Sida spinosa Linn. (Malvaceae) was investigated to verify its claimed ethano medical use in the treatment of microbial infections. Phytochemical analysis revealed the presence of saponins, alkaloids, steroids, flavanoids and glycosides. The test organisms were Staphylococcus aureus, Bacillus subsitlis, Pseudomonas aeroginosa, Escherichia coli, Candida albicans, Aspergillus niger. Amongst the test extracts the results suggested that Ethanolic extract to whole plant of Sida spinosa showed antimicrobial activity.